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Bio::Map::CytoPosition.3pm

NAME

Bio::Map::CytoPosition - Marker class with cytogenetic band storing attributes

SYNOPSIS

   $m1 = Bio::Map::CytoPosition->new ( '-id' => 'A1',
                                        '-value' => '2q1-3'
                                              );
   $m2 = Bio::Map::CytoPosition->new ( '-id' => 'A2',
                                        '-value' => '2q2'
                                              );
 
   if ($m1->cytorange->overlaps($m2->cytorange)) {
       print "Makers overlap\n";
   }
 
 

DESCRIPTION

CytoPosition is marker (Bio::Map::MarkerI compliant) with a location in a cytogenetic map. See Bio::Map::MarkerI for more information.

Cytogenetic locations are names of bands visible in stained mitotic eucaryotic chromosomes. The naming follows strict rules which are consistant at least in higher vertebates, e.g. mammals. The chromosome name preceds the band names.

The shorter arm of the chromosome is called 'p' ('petit') and usually drawn pointing up. The lower arm is called 'q' ('queue'). The bands are named from the region separting these, a centromere (cen), towards the tips or telomeric regions (ter) counting from 1 upwards. Depending of the resolution used the bands are identified with one or more digit. The first digit determines the major band and subsequent digits sub bands: p1 band can be divided into subbands p11, p12 and 13 and p11 can furter be divided into subbands p11.1 and p11.2. The dot after second digit makes it easier to read the values. A region between ands is given from the centromere outwards towards the telomere (e.g. 2p2-5 or 3p21-35) or from a band in the p arm to a band in the q arm.

FEEDBACK

Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing lists Your participation is much appreciated.

   bioperl-l@bioperl.org                  - General discussion
   http://bioperl.org/wiki/Mailing_lists  - About the mailing lists
 
 

Support

Please direct usage questions or support issues to the mailing list:

bioperl-l@bioperl.org

rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

Reporting Bugs

report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web:

   http://bugzilla.open-bio.org/
 
 

AUTHOR - Heikki Lehvaslaiho

Email: heikki-at-bioperl-dot-org

CONTRIBUTORS

Sendu Bala bix@sendu.me.uk

APPENDIX

The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

cytorange

  Title   : cytorange
  Usage   : my $range = $obj->cytorange();
  Function:
             Converts cytogenetic location set by value method into
             an integer range. The chromosome number determines the
             "millions" in the values.  Human X and Y chromosome
             symbols are represented by values 100 and 101.
 
             The localization within chromosomes are converted into
             values between the range of 0 and 200,000:
 
             pter                    cen                       qter
             |------------------------|-------------------------|
             0                     100,000                   200,000
 
             The values between -100,000 through 0 for centromere to
             100,000 would have reflected the band numbering better but
             use of positive integers was choosen since the
             transformation is very easy. These values are not metric.
 
             Each band defines a range in a chromosome. A band string
             is converted into a range by padding it with lower and and
             higher end digits (for q arm: '0' and '9') to the length
             of five. The arm and chromosome values are added to these:
             e.g. 21000 & 21999 (band 21) + 100,000 (q arm) + 2,000,000
             (chromosome 2) => 2q21 : 2,121,000 .. 2,121,999. Note that
             this notation breaks down if there is a band or a subband
             using digit 9 in its name!  This is not the case in human
             karyotype.
 
             The full algorithm used for bands:
 
             if arm is 'q' then
                pad char for start is '0', for end '9'
                range is chromosome + 100,000 + padded range start or end
             elsif arm is 'p' then
                pad char for start is '9', for end '0'
                range is chromosome + 100,000 - padded range start or end
 
  Returns : Bio::Range object or undef
  Args    : none
 
 

range2value

  Title   : range2value
  Usage   : my $value = $obj->range2value($range);
  Function: Sets and returns the value string based on start and end values of
            the Bio::Range object passes as an argument.
  Returns : string or false
  Args    : Bio::Range object
 
 

value

  Title   : value
  Usage   : my $pos = $position->value;
  Function: Get/Set the value for this postion
  Returns : scalar, value
  Args    : none to get, OR scalar to set
 
 

numeric

  Title   : numeric
  Usage   : my $num = $position->numeric;
  Function: Read-only method that is guarantied to return a numeric 
            representation of the start of this position.
  Returns : int (the start of the range)
  Args    : optional Bio::RangeI object
 
 

chr

  Title   : chr
  Usage   : my $mychr = $position->chr();
  Function: Get/Set method for the chromosome string of the location.
  Returns : chromosome value
  Args    : none to get, OR scalar to set