Bio::SeqIO::Handler::GenericRichSeqHandler.3pm

Langue: en

Version: 2010-05-19 (ubuntu - 24/10/10)

Section: 3 (Bibliothèques de fonctions)

NAME

Bio::SeqIO::Handler::GenericRichSeqHandler - Bio::HandlerI-based data handler for GenBank/EMBL/UniProt (and other) sequence data

SYNOPSIS

   # MyHandler is a GenericRichSeqHandler object.
   # inside a parser (driver) constructor....
 
   $self->seq_handler($handler || MyHandler->new(-format => 'genbank'));
 
   # in next_seq() in driver...
 
   $hobj = $self->seqhandler();
 
   # roll data up into hashref chunks, pass off into Handler for processing...
 
   $hobj->data_handler($data);
 
   # or retrieve Handler methods and pass data directly to Handler methods...
 
   my $hmeth = $hobj->handler_methods;
 
   if ($hmeth->{ $data->{NAME} }) {
       my $mth = $hmeth->{ $data->{NAME} };
       $hobj->$mth($data);
   }
 
 

DESCRIPTION

This is an experimental implementation of a sequence-based HandlerBaseI parser and may change over time. It is possible (nay, likely) that the way handler methods are set up will change over development to allow more flexibility. Release pumpkins, please do not add this to a release until the API has settled. It is also likely that write_seq() will not work properly for some data.

Standard Developer caveats:

Do not use for production purposes. Not responsible for destroying (your data|computer|world). Do not stare directly at GenericRichSeqHandler. If GenericRichSeqHandler glows, back slowly away and call for help.

Consider yourself warned!

This class acts as a demonstration on how to handle similar data chunks derived from Bio::SeqIO::gbdriver, Bio::SeqIO::embldriver, and Bio::SeqIO::swissdriver using similar (or the same) handler methods.

The modules currently pass all previous tests in t/genbank.t, t/embl.t, and t/swiss.t yet all use the same handler methods (the collected tests for handlers can be found in t/Handler.t). Some tweaking of the methods themselves is probably in order over the long run to ensure that data is consistently handled for each parser. Round-trip tests are probably in order here...

Though a Bio::Seq::SeqBuilder is employed for building sequence objects no bypassing of data based on builder slots has been implemented (yet); this is planned in the near future.

As a reminder: this is the current Annotation data chunk (via Data::Dumper):

   $VAR1 = {
             'NAME' => 'REFERENCE',
             'DATA' => '1  (bases 1 to 10001)'
             'AUTHORS' => 'International Human Genome Sequencing Consortium.'
             'TITLE' => 'The DNA sequence of Homo sapiens'
             'JOURNAL' => 'Unpublished (2003)'
           };
   ...
 
 

This is the current SeqFeature data chunk (again via Data::Dumper):

   $VAR1 = {
             'mol_type' => 'genomic DNA',
             'LOCATION' => '<1..>10001',
             'NAME' => 'FEATURES',
             'FEATURE_KEY' => 'source',
             'note' => 'Accession AL451081 sequenced by The Sanger Centre',
             'db_xref' => 'taxon:9606',
             'clone' => 'RP11-302I18',
             'organism' => 'Homo sapiens'
           };
 
 

FEEDBACK

Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated.
   bioperl-l@bioperl.org                  - General discussion
   http://bioperl.org/wiki/Mailing_lists  - About the mailing lists
 
 

Support

Please direct usage questions or support issues to the mailing list:

bioperl-l@bioperl.org

rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web:
   http://bugzilla.open-bio.org/
 
 

AUTHOR - Chris Fields

Email cjfields at bioperl dot org

APPENDIX

The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

new

  Title   :  new
  Usage   :  
  Function:  
  Returns :  
  Args    :  -format    Sequence format to be mapped for handler methods
             -builder   Bio::Seq::SeqBuilder object (normally defined in
                        SequenceStreamI object implementation constructor)
  Throws  :  On undefined '-format' sequence format parameter
  Note    :  Still under heavy development
 
 

Bio::HandlerBaseI implementing methods

handler_methods

  Title   :  handler_methods
  Usage   :  $handler->handler_methods('GenBank')
             %handlers = $handler->handler_methods();
  Function:  Retrieve the handler methods used for the current format() in
             the handler.  This assumes the handler methods are already
             described in the HandlerI-implementing class.
  Returns :  a hash reference with the data type handled and the code ref
             associated with it.
  Args    :  [optional] String representing the sequence format.  If set here
             this will also set sequence_format()
  Throws  :  On unimplemented sequence format in %HANDLERS
 
 

data_handler

  Title   :  data_handler
  Usage   :  $handler->data_handler($data)
  Function:  Centralized method which accepts all data chunks, then distributes
             to the appropriate methods for processing based on the chunk name
             from within the HandlerBaseI object.
 
             One can also use 
  Returns :  None
  Args    :  an hash ref containing a data chunk.
 
 

reset_parameters

  Title   :  reset_parameters
  Usage   :  $handler->reset_parameters()
  Function:  Resets the internal cache of data (normally object parameters for
             a builder or factory)
  Returns :  None
  Args    :  None
 
 

format

  Title   :  format
  Usage   :  $handler->format('GenBank')
  Function:  Get/Set the format for the report/record being parsed. This can be
             used to set handlers in classes which are capable of processing
             similar data chunks from multiple driver modules.
  Returns :  String with the sequence format
  Args    :  [optional] String with the sequence format
  Note    :  The format may be used to set the handlers (as in the
             current GenericRichSeqHandler implementation)
 
 

get_params

  Title   :  get_params
  Usage   :  $handler->get_params('-species')
  Function:  Convenience method used to retrieve the specified
             parameters from the internal parameter cache
  Returns :  Hash ref containing parameters requested and data as
             key-value pairs.  Note that some parameter values may be 
             objects, arrays, etc.
  Args    :  List (array) representing the parameters requested
 
 

set_params

  Title   :  set_params
  Usage   :  $handler->set_param({'-species')
  Function:  Convenience method used to set specific parameters
  Returns :  None
  Args    :  Hash ref containing the data to be passed as key-value pairs
 
 

Methods unique to this implementation

seqbuilder

  Title   :  seqbuilder
  Usage   :  
  Function:  
  Returns :  
  Args    :
  Throws  :
  Note    :
 
 

build_sequence

  Title   :  build_sequence
  Usage   :  
  Function:  
  Returns :  
  Args    :
  Throws  :
  Note    :
 
 

location_factory

  Title   :  location_factory
  Usage   :  
  Function:  
  Returns :  
  Args    :
  Throws  :
  Note    :
 
 

annotation_collection

  Title   :  annotation_collection
  Usage   :  
  Function:  
  Returns :  
  Args    :
  Throws  :
  Note    :