Bio::SeqIO::scf.3pm

Langue: en

Version: 2010-05-19 (ubuntu - 24/10/10)

Section: 3 (Bibliothèques de fonctions)

NAME

Bio::SeqIO::scf - .scf file input/output stream

SYNOPSIS

Do not use this module directly. Use it via the Bio::SeqIO class, see Bio::SeqIO for more information.

DESCRIPTION

This object can transform .scf files to and from Bio::Seq::SequenceTrace objects. Mechanisms are present to retrieve trace data from scf files.

FEEDBACK

Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated.
   bioperl-l@bioperl.org                  - General discussion
   http://bioperl.org/wiki/Mailing_lists  - About the mailing lists
 
 

Support

Please direct usage questions or support issues to the mailing list:

bioperl-l@bioperl.org

rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web:
   http://bugzilla.open-bio.org/
 
 

AUTHOR Chad Matsalla

Chad Matsalla bioinformatics@dieselwurks.com

CONTRIBUTORS

Jason Stajich, jason@bioperl.org Tony Cox, avc@sanger.ac.uk Heikki Lehvaslaiho, heikki-at-bioperl-dot-org Nancy Hansen, nhansen at mail.nih.gov

APPENDIX

The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

next_seq()

  Title   : next_seq()
  Usage   : $scf = $stream->next_seq()
  Function: returns the next scf sequence in the stream
  Returns : a Bio::Seq::SequenceTrace object
  Args    : NONE
  Notes   : Fills the interface specification for SeqIO.
             The SCF specification does not provide for having more then
            one sequence in a given scf. So once the filehandle has been open
            and passed to SeqIO do not expect to run this function more then
            once on a given scf unless you embraced and extended the SCF
        standard.  SCF comments are accessible through the Bio::SeqI
            interface method annotation().
 
 

_get_v3_quality()

  Title   : _get_v3_quality()
  Usage   : $self->_get_v3_quality()
  Function: Set the base qualities from version3 scf
  Returns : Nothing. Alters $self.
  Args    : None.
  Notes   :
 
 

_get_v3_peak_indices($buffer)

  Title   : _get_v3_peak_indices($buffer)
  Usage   : $self->_get_v3_peak_indices($buffer);
  Function: Unpacks the base accuracies for version3 scf
  Returns : Nothing. Alters $self
  Args    : A scalar containing binary data.
  Notes   :
 
 

_get_v3_base_accuracies($buffer)

  Title   : _get_v3_base_accuracies($buffer)
  Usage   : $self->_get_v3_base_accuracies($buffer)
  Function: Set the base accuracies for version 3 scf's
  Returns : Nothing. Alters $self.
  Args    : A scalar containing binary data.
  Notes   :
 
 

_get_comments($buffer)

  Title   : _get_comments($buffer)
  Usage   : $self->_get_comments($buffer);
  Function: Gather the comments section from the scf and parse it into its
             components.
  Returns : a Bio::Annotation::Collection object
  Args    : The buffer. It is expected that the buffer contains a binary
             string for the comments section of an scf file according to
             the scf file specifications.
  Notes   :
 
 

_get_header()

  Title   : _get_header($buffer)
  Usage   : $self->_get_header($buffer);
  Function: Gather the header section from the scf and parse it into its
            components.
  Returns : Reference to a hash containing the header components.
  Args    : The buffer. It is expected that the buffer contains a binary
            string for the header section of an scf file according to the
            scf file specifications.
  Notes   : None.
 
 

_parse_v2_bases($buffer)

  Title   : _parse_v2_bases($buffer)
  Usage   : $self->_parse_v2_bases($buffer);
  Function: Gather the bases section from the scf and parse it into its
            components.
  Returns :
  Args    : The buffer. It is expected that the buffer contains a binary
            string for the bases section of an scf file according to the
            scf file specifications.
  Notes   : None.
 
 

_parse_v2_traces(\@traces_array)

  Title   : _pares_v2_traces(\@traces_array)
  Usage   : $self->_parse_v2_traces(\@traces_array);
  Function: Parses an scf Version2 trace array into its base components.
  Returns : Nothing. Modifies $self.
  Args    : A reference to an array of the unpacked traces section of an
            scf version2 file.
 
 

get_header()

  Title   : get_header()
  Usage   : %header = %{$obj->get_header()};
  Function: Return the header for this scf.
  Returns : A reference to a hash containing the header for this scf.
  Args    : None.
  Notes   :
 
 

get_comments()

  Title   : get_comments()
  Usage   : %comments = %{$obj->get_comments()};
  Function: Return the comments for this scf.
  Returns : A Bio::Annotation::Collection object
  Args    : None.
  Notes   :
 
 

write_seq

  Title   : write_seq(-target => $swq, <comments>)
  Usage   : $obj->write_seq(
                -target => $swq,
             -version => 2,
             -CONV => "Bioperl-Chads Mighty SCF writer.");
  Function: Write out an scf.
  Returns : Nothing.
  Args    : Requires: a reference to a Bio::Seq::Quality object to form the
            basis for the scf.
        if -version is provided, it should be "2" or "3". A SCF of that
        version will be written.
        Any other arguments are assumed to be comments and are put into
        the comments section of the scf. Read the specifications for scf
        to decide what might be good to put in here.
 
  Notes   :
           For best results, use a SequenceTrace object.
           The things that you need to write an scf:
           a) sequence
           b) quality
           c) peak indices
           d) traces
           - You _can_ write an scf with just a and b by passing in a
                Bio::Seq::Quality object- false traces will be synthesized
                for you.
 
 

_get_binary_header()

  Title   : _get_binary_header();
  Usage   : $self->_get_binary_header();
  Function: Provide the binary string that will be used as the header for
             a scfv2 document.
  Returns : A binary string.
  Args    : None. Uses the entries in the $self->{'header'} hash. These
             are set on construction of the object (hopefully correctly!).
  Notes   :
 
 

_get_binary_traces($version,$ref)

  Title   : _set_binary_tracesbases($version,$ref)
  Usage   : $self->_set_binary_tracesbases($version,$ref);
  Function: Constructs the trace and base strings for all scfs
  Returns : Nothing. Alters self.
  Args    : $version - "2" or "3"
        $sequence - a scalar containing arbitrary sequence data
        $ref - a reference to either a SequenceTraces or a
           SequenceWithQuality object.
  Notes   : This is a really complicated thing.
 
 

_make_trace_string($version)

  Title   : _make_trace_string($version)
  Usage   : $self->_make_trace_string($version)
  Function: Merges trace data for the four bases to produce an scf
        trace string. _requires_ $version
  Returns : Nothing. Alters $self.
  Args    : $version - a version number. "2" or "3"
  Notes   :
 
 

_get_binary_comments(\@comments)

  Title   : _get_binary_comments(\@comments)
  Usage   : $self->_get_binary_comments(\@comments);
  Function: Provide a binary string that will be the comments section of
        the scf file. See the scf specifications for detailed
        specifications for the comments section of an scf file. Hint:
        CODE=something\nBODE=something\n\0
  Returns :
  Args    : A reference to an array containing comments.
  Notes   : None.
 
 

_delta(\@trace_data,$direction)

  Title   : _delta(\@trace_data,$direction)
  Usage   : $self->_delta(\@trace_data,$direction);
  Function:
  Returns : A reference to an array containing modified trace values.
  Args    : A reference to an array containing trace data and a string
        indicating the direction of conversion. ("forward" or
        "backward").
  Notes   : This code is taken from the specification for SCF3.2.
        http://www.mrc-lmb.cam.ac.uk/pubseq/manual/formats_unix_4.html
 
 

_unpack_magik($buffer)

  Title   : _unpack_magik($buffer)
  Usage   : $self->_unpack_magik($buffer)
  Function: What unpack specification should be used? Try them all.
  Returns : Nothing.
  Args    : A buffer containing arbitrary binary data.
  Notes   : Eliminate the ambiguity and the guesswork. Used in the
        adaptation of _delta(), mostly.
 
 

read_from_buffer($filehandle,$buffer,$length)

  Title   : read_from_buffer($filehandle,$buffer,$length)
  Usage   : $self->read_from_buffer($filehandle,$buffer,$length);
  Function: Read from the buffer.
  Returns : $buffer, containing a read of $length
  Args    : a filehandle, a buffer, and a read length
  Notes   : I just got tired of typing
        "unless (length($buffer) == $length)" so I put it here.
 
 

_dump_keys()

  Title   : _dump_keys()
  Usage   : &_dump_keys($a_reference_to_some_hash)
  Function: Dump out the keys in a hash.
  Returns : Nothing.
  Args    : A reference to a hash.
  Notes   : A debugging method.
 
 

_dump_base_accuracies()

  Title   : _dump_base_accuracies()
  Usage   : $self->_dump_base_accuracies();
  Function: Dump out the v3 base accuracies in an easy to read format.
  Returns : Nothing.
  Args    : None.
  Notes   : A debugging method.
 
 

_dump_peak_indices_incoming()

  Title   : _dump_peak_indices_incoming()
  Usage   : $self->_dump_peak_indices_incoming();
  Function: Dump out the v3 peak indices in an easy to read format.
  Returns : Nothing.
  Args    : None.
  Notes   : A debugging method.
 
 

_dump_base_accuracies_incoming()

  Title   : _dump_base_accuracies_incoming()
  Usage   : $self->_dump_base_accuracies_incoming();
  Function: Dump out the v3 base accuracies in an easy to read format.
  Returns : Nothing.
  Args    : None.
  Notes   : A debugging method.
 
 

_dump_comments()

  Title   : _dump_comments()
  Usage   : $self->_dump_comments();
  Function: Debug dump the comments section from the scf.
  Returns : Nothing.
  Args    : Nothing.
  Notes   : None.